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human siglec  (R&D Systems)


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Bioz Manufacturer Symbol R&D Systems manufactures this product  
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    Structured Review

    R&D Systems human siglec
    Human Siglec, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mag+fc/Recombinant+Human+MAG%2FSiglec-4a+Fc+Chimera+Protein%2C+CF/pm38354234-367-37-41
    Average 92 stars, based on 3 article reviews
    human siglec - by Bioz Stars, 2026-10
    92/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Deacetylation of Miro1 by HDAC6 blocks mitochondrial transport and mediates axon growth inhibition
    Article Snippet: .. For bath application, dissociated DRGs were cultured for 48 h and treated with 25 μg/ml of MAG-Fc (R&D Systems; 538-MG), 25 μg/ml Fc control (R&D Systems; 110-HG), or 10 μg/ml of Aggrecan (Sigma-Aldrich; A1960) for 4 h. Cultures were pretreated with either Ca 2+ chelator BAPTA-AM (3 μM; Sigma-Aldrich; A1076), ROCK inhibitor Y27632 (10 μM; Sigma-Aldrich; SCM075), HDAC6 inhibitor TubA (10 μM; R&D Systems; 6270), Thapsigargin (1 μM; Sigma-Aldrich; T9033), Rho Activator II (1 μg/ml; Cytoskeleton; CN03), or equivalent volume DMSO 30 min before MAG-Fc, Aggrecan, or controls. .. For substrate-bound experiments, adult DRGs were cultured on glass bottom dishes (Greiner) that were coated with 25 μg/ml Aggrecan in addition to the laminin/poly- d -lysine substrate.

    Control:

    Article Title: Deacetylation of Miro1 by HDAC6 blocks mitochondrial transport and mediates axon growth inhibition
    Article Snippet: .. For bath application, dissociated DRGs were cultured for 48 h and treated with 25 μg/ml of MAG-Fc (R&D Systems; 538-MG), 25 μg/ml Fc control (R&D Systems; 110-HG), or 10 μg/ml of Aggrecan (Sigma-Aldrich; A1960) for 4 h. Cultures were pretreated with either Ca 2+ chelator BAPTA-AM (3 μM; Sigma-Aldrich; A1076), ROCK inhibitor Y27632 (10 μM; Sigma-Aldrich; SCM075), HDAC6 inhibitor TubA (10 μM; R&D Systems; 6270), Thapsigargin (1 μM; Sigma-Aldrich; T9033), Rho Activator II (1 μg/ml; Cytoskeleton; CN03), or equivalent volume DMSO 30 min before MAG-Fc, Aggrecan, or controls. .. For substrate-bound experiments, adult DRGs were cultured on glass bottom dishes (Greiner) that were coated with 25 μg/ml Aggrecan in addition to the laminin/poly- d -lysine substrate.

    Recombinant:

    Article Title: Neutralizing monoclonal antibodies against the Nogo-66 receptor (NgR) and uses thereof
    Article Snippet: We immobilized NgR-Fc (0.2 μg/well, R&D Systems) on 96-well microtiter plates (Nunc Maxisorb) in 50 mM Na-carbonate buffer pH 9 over night at 4° C. followed by a 2 h blocking step with 2% BSA in Tris-HCL, pH 7.2 at room temperature. .. MAG-Fc (R&D Systems recombinant Rat MAG/Fc Chimera, Catalog #538-MG) was labeled with horseradish peroxidase by the Zenon human IgG labeling kit (Molecular Probes). ..

    Labeling:

    Article Title: Neutralizing monoclonal antibodies against the Nogo-66 receptor (NgR) and uses thereof
    Article Snippet: We immobilized NgR-Fc (0.2 μg/well, R&D Systems) on 96-well microtiter plates (Nunc Maxisorb) in 50 mM Na-carbonate buffer pH 9 over night at 4° C. followed by a 2 h blocking step with 2% BSA in Tris-HCL, pH 7.2 at room temperature. .. MAG-Fc (R&D Systems recombinant Rat MAG/Fc Chimera, Catalog #538-MG) was labeled with horseradish peroxidase by the Zenon human IgG labeling kit (Molecular Probes). ..



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